RESUMEN
BACKGROUND: Gene expression profiling of normal receptive endometrium has been characterized, but intrinsic defects in endometrial gene expression associated with implantation failure have not been reported. METHODS: Women who had previously participated as recipients in oocyte donation cycles and repeatedly exhibited implantation failure (Group A, study group) or had at least one successful cycle (Group B, control group) and spontaneously fertile women (Group C, normal fertility group) were recruited. All were treated with exogenous estradiol and progesterone to induce an endometrial cycle, and an endometrial biopsy was taken on the seventh day of progesterone administration. RNA from each sample was analysed by cDNA microarrays to identify differentially expressed genes between groups. RESULTS: 63 transcripts were differentially expressed (>or=2-fold) between Groups A and B, of which 16 were subjected to real time RT-PCR. Eleven of these were significantly decreased in Group A with regard to Groups B and C. Among the dysregulated genes were MMP-7, CXCR4, PAEP and C4BPA. CONCLUSIONS: Repeated implantation failure in some oocyte recipients is associated with an intrinsic defect in the expression of multiple genes in their endometrium. Significantly decreased levels of several transcripts in endometria without manifest abnormalities is demonstrated for the first time and shown to be associated with implantation failure.
Asunto(s)
Implantación del Embrión , Endometrio/metabolismo , Perfilación de la Expresión Génica , Embarazo , ARN Mensajero/metabolismo , Adulto , Proteína de Unión al Complemento C4b , Femenino , Glicodelina , Glicoproteínas/genética , Antígenos de Histocompatibilidad/genética , Humanos , Metaloproteinasa 7 de la Matriz/genética , Persona de Mediana Edad , Análisis de Secuencia por Matrices de Oligonucleótidos , Proteínas Gestacionales/genética , Receptores CXCR4/genética , Reacción en Cadena de la Polimerasa de Transcriptasa InversaRESUMEN
Se presenta el método para obtener rápidamente, mediante un microcomputador y software específico, los gráficos de curvas hormonales promedio de hormona luteinizante (LH) de 15 ciclos concepcionales, en los que se determinan los valores plasmáticos de LH, de hormona folículo-estimulante (FSH), de prolactina (PRL), de estradiol (E2) y progesterona (P). Se establecen las ventajes para el investigador del uso de un sistema de informática personal
